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1.
Guinea pig pancreatic slice in vitro EMAR 3 min. pulse

Creator:
Jamieson, James D
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides, Original Magnification: x17,000, and Pulse chase experiments, carried out by Jamieson and Palade, defined the kinetics and pathway of intracellular transport of newly synthesized proteins in guinea pig pancreatic slices in vitro. The 3 min pulse consisted of preincubation in the cold in a leucine-free medium containing tritiated leucine, followed by warming up to 37 for 3 min. Chase incubations followed by incubation in medium containing an excess of "cold" leucine, fixation at indicated times, and processing for EM autoradiography. Historic details in the Nobel Lecture 1974 by Dr. Palade.
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 3 min. pulse

2.
Guinea pig pancreatic slice in vitro EMAR 3 min. pulse + 117 min. chase

Creator:
Jamieson, James D
Published / Created:
2/2/1964
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides and Original Magnification: x17,000
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 3 min. pulse + 117 min. chase

3.
Guinea pig pancreatic slice in vitro EMAR 3 min. pulse + 37 min. chase

Creator:
Jamieson, James D
Published / Created:
11/11/1972
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides and Original Magnification: x17,000
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 3 min. pulse + 37 min. chase

4.
Guinea pig pancreatic slice in vitro EMAR 3 min. pulse + 7 min. chase

Creator:
Jamieson, James D
Published / Created:
1/1/1962
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides and Original Magnification: x17,000
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 3 min. pulse + 7 min. chase

5.
Guinea pig pancreatic slice in vitro EMAR 3 min. pulse+ 117 min. chase duct lumen

Creator:
Jamieson, James D
Published / Created:
2/2/1964
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides and Original Magnification: x17,000
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 3 min. pulse+ 117 min. chase duct lumen

6.
Guinea pig pancreatic slice in vitro EMAR 3 min. pulse+ 37 min. chase zymogen granule fraction

Creator:
Jamieson, James D
Published / Created:
1968
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides, Original Magnification: x1,000, and Zymogen granule fractions were isolated from pancreatic slices pulse labeled and incubated in vitro. Processing for EMAR was as for tissues.
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 3 min. pulse+ 37 min. chase zymogen granule fraction

7.
Guinea pig pancreatic slice in vitro EMAR 3 min. pulse+ 37 min. chase zymogen granule fraction

Creator:
Jamieson, James D
Published / Created:
9/20/1963
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides and Original Magnification: x13,000
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 3 min. pulse+ 37 min. chase zymogen granule fraction

8.
Guinea pig pancreatic slice in vitro EMAR 3min pulse + 17 min chase after 180 min stimulation 0.01

Creator:
Jamieson, James D
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides and Original Magnification: x10,000
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice stimulated in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 3min pulse + 17 min chase after 180 min stimulation 0.01 mM carbachol

9.
Guinea pig pancreatic slice in vitro EMAR 3min pulse + 7 min chase after 180 min stimulation 0.01

Creator:
Jamieson, James D
Published / Created:
1/1/1945
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides and Original Magnification: x10,000
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice stimulated in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 3min pulse + 7 min chase after 180 min stimulation 0.01 mM carbachol

10.
Guinea pig pancreatic slice in vitro EMAR 3min pulse +37 chase with cycloheximide

Creator:
Jamieson, James D
Published / Created:
5/6/1967
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides, Original Magnification: x10,000, and Protein transport from the RER to the final steps of exocytosis does not require ongoing protein synthesis as determined in these studies. We postulated that synthesis of new proteins such as membrane proteins, in the short term, is not required. Recycled zymogen granule membrane may provide the needed reservoir of membranes for construction of new zymogen granules.
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 3min pulse +37 chase with cycloheximide

11.
Guinea pig pancreatic slice in vitro EMAR 3min pulse +57 chase

Creator:
Jamieson, James D
Published / Created:
2/2/1966
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides and Original Magnification: x10,000
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 3min pulse +57 chase

12.
Guinea pig pancreatic slice in vitro EMAR 3min pulse +57 chase with antimycin A and cycloheximide

Creator:
Jamieson, James D
Published / Created:
5/12/1966
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides, Intracellular transport from the RER to the Golgi periphery requires ATP but not continued protein synthesis. The evidence suggested that the energy-requiring step may be located at the transitional elements of the RER and may be involved in the pinching off of Golgi transport vesicles., and Original Magnification: x10,000
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 3min pulse +57 chase with antimycin A and cycloheximide

13.
Guinea pig pancreatic slice in vitro EMAR 3min pulse after 180 min stimulation 0.01 mM carbachol

Creator:
Jamieson, James D
Published / Created:
11/11/1972
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides, Original Magnification: x12,000, and Same as previous micrograph of prestimulated pancreatic slices.
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice stimulated in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 3min pulse after 180 min stimulation 0.01 mM carbachol

14.
Guinea pig pancreatic slice in vitro EMAR 3min pulse after 180 min stimulation with 0.01mM

Creator:
Jamieson, James D
Published / Created:
12/12/1963
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides and These studies showed that the rate of protein synthesis, rate of drainage of the RER to the Golgi and the overall transit rate over the secretory pathway was not accelerated by prior secretagogue release of all preformed zymogen granules. Furthermore, secretory proteins were not rerouted into the cytosol as had been proposed by others.
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice stimulated in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 3min pulse after 180 min stimulation with 0.01mM carbachol

15.
Guinea pig pancreatic slice in vitro EMAR 4 min. pulse + 80 min. chase

Creator:
Jamieson, James D
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides, Original Magnification: x16,000, and Protein synthesis is not required for any step in the secretory pathway beyond initial synthesis of exportable proteins. ATP is need for RER to Golgi transport, not for concentration, but is required for stimulated exocytosis.
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 4 min. pulse + 80 min. chase

16.
Guinea pig pancreatic slice in vitro EMAR 4 min. pulse+ 20 min. chase

Creator:
Jamieson, James D
Published / Created:
2/1/1966
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides and Original Magnification: x13,000
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreatic slice in vitro EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreatic slice in vitro EMAR 4 min. pulse+ 20 min. chase

17.
Single guinea pig pancreatic acinar cells 10 min pulse +4 hr chase EMAR

Creator:
Amsterdam. A
Jamieson, James D
Published / Created:
9/15/1965
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides and Original Magnification: x6,000
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Single guinea pig pancreatic acinar cells
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Single guinea pig pancreatic acinar cells 10 min pulse +4 hr chase EMAR

19.
Guinea pig pancreas in vivo EMAR 20 min post injection

Creator:
Caro, Lucien
Published / Created:
2/2/1958
Image Count:
1
Resource Type:
Prints & Photographs
Description:
3.25 in. x 4 in. Lantern Slides and Original Magnification: x30,000
Publisher:
Yale University School of Medicine
Subject (Name):
Jamieson, James D
Subject (Topic):
Guinea pig pancreas in vivo EMAR
Found in:
Medical Historical Library, Cushing/Whitney Medical Library > Guinea pig pancreas in vivo EMAR 20 min post injection