3.25 in. x 4 in. Lantern Slides, Original Magnification: x17,000, and Pulse chase experiments, carried out by Jamieson and Palade, defined the kinetics and pathway of intracellular transport of newly synthesized proteins in guinea pig pancreatic slices in vitro. The 3 min pulse consisted of preincubation in the cold in a leucine-free medium containing tritiated leucine, followed by warming up to 37 for 3 min. Chase incubations followed by incubation in medium containing an excess of "cold" leucine, fixation at indicated times, and processing for EM autoradiography. Historic details in the Nobel Lecture 1974 by Dr. Palade.
3.25 in. x 4 in. Lantern Slides, Original Magnification: x1,000, and Zymogen granule fractions were isolated from pancreatic slices pulse labeled and incubated in vitro. Processing for EMAR was as for tissues.
3.25 in. x 4 in. Lantern Slides, Original Magnification: x10,000, and Protein transport from the RER to the final steps of exocytosis does not require ongoing protein synthesis as determined in these studies. We postulated that synthesis of new proteins such as membrane proteins, in the short term, is not required. Recycled zymogen granule membrane may provide the needed reservoir of membranes for construction of new zymogen granules.
3.25 in. x 4 in. Lantern Slides, Intracellular transport from the RER to the Golgi periphery requires ATP but not continued protein synthesis. The evidence suggested that the energy-requiring step may be located at the transitional elements of the RER and may be involved in the pinching off of Golgi transport vesicles., and Original Magnification: x10,000
